Molecular identification of Antarctic canola oil-degrading bacteria
Ahmad, Siti Aqlima
- 1Universiti Putra Malaysia
- 2
- 3Universiti Teknologi MARA
- 4British Antarctic Survey
- 5Shibaura Institute of Technology
- 6
Journal
Polish Polar Research
ISSN
2081-8262
0138-0338
Open Access
diamond
Volume
46
Start page
167
End page
184
The detrimental impacts of human activity in Antarctica can pose significant threats to the continent's ecosystems and diversity. Various pollutants have been detected in Antarctica, including hydrocarbon compounds derived from oils. The widespread use of canola oil in catering at Antarctic research stations raises the possibility of its release into the environment, either through accidental spillage or via waste cooking oil present in grey water. To help address this issue, a bacterial consortium (reference BS14) obtained from the natural Antarctic environment was isolated and confirmed to be capable of breaking down canola oil. The identity of members of the consortium was investigated using metagenomic analysis, with lipase-producing bacteria further examined using Sanger sequencing. The consortium in media not exposed to canola oil consisted primarily of Proteobacteria and Firmicutes in almost equal percentages. After being exposed to either fresh or waste canola oil, high proportions of representatives of Pseudomonadaceae and Carnobacteriaceae were present. Amongst the bacterial taxa identified in the metagenomic analysis, representatives of the genera Pseudomonas and Carnobacterium were confirmed to be responsible for biodegrading waste canola oil and pure canola oil. This study offers novel insights into the potential of bacterial consortia for canola oil bioremediation in Antarctica.
Name
2025-03-PPR-03.pdf
Type
Main Article
Size
1.8 MB
Format
Adobe PDF
Checksum
(MD5):98bbdc87d5cdfb97ec9149fca3a14b7d
Attribution 4.0 International