Chiroptical characterization of homopolymeric block fractions in alginates
Troncoso-Valenzuela, Marcos A.
- 1Universidad de Santiago de Chile
- 2
- 3University of Buenos Aires
- 4Universidad de Concepcion
Journal
Carbohydrate Polymers
ISSN
0144-8617
1879-1344
Open Access
bronze
Volume
146
Start page
90
End page
101
Homopolymannuronic and homopolyguluronic fractions were obtained by partial hydrolysis of the alkaline extracts from the brown seaweeds Ascoseira mirabilis, Desmarestia menziessi, Desmarestia ligulata and Durvillaea sp. collected in southern Chile. Full characterization of the fractions was achieved by FT-IR and NMR spectroscopy. Total hydrolysis with 90% formic acid of the homopolymeric fractions allowed the preparation of mannuronic and guluronic acids. Both monomers and homopolymeric fractions as neutral salts were studied by CD and ORD. Chiroptical spectra were similar in shape and sign to those previously published in the literature, and permitted to assign D configuration to mannuronic acid and L configuration to guluronic acid in alginic acids. Specific optical rotation values at the sodium D light for the homopolymannuronic (similar to 100 degrees) and homopolyguluronic (similar to 110 degrees) acid fractions were obtained. These high negative values are proposed for the assignment of the absolute configuration of monomers in homopolymeric fractions. (C) 2016 Elsevier Ltd. All rights reserved.