Continuous biohydrogen production by a degenerated strain of Clostridium acetobutylicum ATCC 824
Guerrero, Karlo
Pontificia Universidad Catolica de Valparaiso
Paredes, Ivan
Pontificia Universidad Catolica de Valparaiso
Quintero, Julian
Universidad de Santiago de Chile
Mau, Silvia
Pontificia Universidad Catolica de Valparaiso
Conejeros, Raul
Pontificia Universidad Catolica de Valparaiso
Carlos Gentina, Juan
Pontificia Universidad Catolica de Valparaiso
Aroca, German
Pontificia Universidad Catolica de Valparaiso
Journal
international journal of hydrogen energy
ISSN
0360-3199
1879-3487
Open Access
closed
Volume
46
Start page
5100
End page
5111
Degenerated strains of Clostridium acetobutylicum lack the ability to produce solvents and to sporulate, allowing the continuous production of hydrogen and organic acids. A degenerated strain of Clostridium acetobutylicum was obtained through successive batch cultures. Its kinetic characterization showed a similar specific growth rate than the wild type (0.25 h(-1)), a higher butyric acid production of 6.8 g-L-1 and no solvents production. A steady state was reached in a continuous culture at a dilution rate of 0.1 h(-1), with a constant hydrogen production of 507 mL.h(-1), corresponding to a volumetric rate of 6.10 L.L(-1)d(-1), and a yield of 2.39 mol of H-2 per mole of glucose which represents 60% of the theoretical maximum yield. These results suggest that the degeneration is an interesting alternative for hydrogen production with this strain, obtaining a high hydrogen production in a continuous culture with cells in a permanent acidogenic state. (C) 2020 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.